Coding

Part:BBa_K3594014:Design

Designed by: Runhan Luo   Group: iGEM20_SHSBNU_China   (2020-10-23)


Cytochrome P450 gene (CYP)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 315
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 163
    Illegal NgoMIV site found at 288
    Illegal NgoMIV site found at 763
    Illegal NgoMIV site found at 1039
    Illegal NgoMIV site found at 1057
    Illegal AgeI site found at 399
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We use this part with Ferredoxin Reductase together to function as the guaiacol degrading enzyme system.


Source

https://amb-express.springeropen.com/articles/10.1186/s13568-019-0759-8#Tab1

References